Aside from RE.

Dante - Devil May Cry
Solid Snake - Metal Gear Solid
Samus Aran (Hot) - Metroid
Link - Legend of Zelda
Zelda - Legend of Zelda
Cloud - Final Fantasy
Sephiroth - Final Fantasy
Auron - Final Fantasy
Rikku - Final Fantasy
Mario - Super Mario
Nathan Drake - Uncharted
Kratos - God of War
Kairi (Cute) - Kingdom Hearts
Riku - Kingdom Hearts
Sora - Kingdom Hearts

I was gonna make this thread but you beat me to it

Black Mage - FFI
Kefka - FFVI
Cloud Strife & Sephiroth - FFVII
Nathan Drake - Uncharted
Solid Snake - Metal Gear
Scorpion & Johnny Cage - Mortal Kombat
Dan & Akuma - Street Fighter
Niko Bellic - GTAIV
Vanessa Z. Schneider - P.N.03 (just for the cool moves)
Adell & Axel - Disgaea 2
Ryu Hayabusa - Ninja Gaiden
Felicia - Darkstalkers
Taokaka, Hakumen & Bang Shishigami - BlazBlue
Henry Townshend & Alex Shepherd - Silent Hill
Tanner - Driver
Scott Pilgrim - Scott Pilgrim vs. The World
Frank West - Dead Rising (still not enough to make me wanna buy Off the Record)
The Belmont Family - Castlevania
Fiolina Germi, Hyakutaro Ichimonji and Super Vehicle 001 - Metal Slug
Fuchikoma/Tachikoma & Togusa - Ghost in the Shell
"Azure Dragon" Date Masamune - Sengoku Basara
Yuri Volte Hyuga - Shadow Hearts
GLaDOS - Portal
Nina Loco (w/ El Guerrero) - V8: 2nd Offense
Crash bandicoot
Viewtiful Joe
Max Payne
Duke Nukem

I have a lot of them, but all af these guys are awesome. They would make the greatest mix of heroes.

Issac Clarke-Dead Space 1,2
Dante-Devil May Cry 1,2,3,4
Leon-Resident Evil 2,4

Can't think of any others.

Captain Price - COD4, MW2
Nico Belic - GTA4
Max Payne, Vladimir Lem - MP games
Reptile, Johnny Cage, Goro, Shao Kahn, Sonya, Jade, Sindel - MK
Ethan Thomas (VERY badass), Rosa, Black Bum, Serial Killer X (this guy kills cops and other serial killers lol) - Condemned 1 and 2
Ryo, Joy, Ren, Lan Di, Jianmin Tao - Shenmue 1 and 2
Tab, Rukakku Goji, Noise Tanks, Gum, Cube, Poison Jam - JGR and JSRF

Jill- RE
Chris-RE
Wesker- RE
Albedo- Xenosaga
Link- Legend of Zelda
Zelda- Legend of Zelda
Midna- Legend of Zelda
Ada- RE
Dante- Devil May Cry
Ezio- Assassin's Creed 2
Cloud- Final Fantasy VII
Rikku- Final Fantasy X and X-2
Yuffi- Final Fantasy VII
Auron- Final Fantasy X
Lightning- Final Fantasy XIII
Vanille- Final Fantasy XIII
Snow- Final Fantasy XIII
Scott Pilgrim- Scott Pilgrim Vs. The World
Riku- Kingdom Hearts
Axel- Kingdom Hearts
Leon- RE
Mast Chief- Halo

That is all I can think of for the life of me at the moment.

Guys, it was supposed to be ASIDE from RE

Ooh, I forgot Johnny Cage. Added.


EDIT: How could I have forgotten Hakumen? The most awesome video game character ever! Also bumping this thread.

I forgot Auron and Rikku from FFX.

HUNK - Resident Evil (sorry I had to lol)
Three Dog - Fallout 3
Pyramid Head - Silent Hill
Ethan Thomas - Condemned 2: Bloodshot
Niko Bellic - GTA4
Francis - Left 4 Dead
Scout - Team Fortess 2
Duke Nukem, he's so badass I don't even need to name one of his games.

Dang it! Aside from RE!? Wow... this is going to be a sucky list lol

General RAAM - Gears of War
Damon Baird - Gears of War
Kabal - Mortal Kombat
Waluigi - Mario
Crash Bandicoot - Duh, Crash Bandicoot
*coughWesker-ResidentEvilcough*
Samus Aran - Metroid
Naked Snake - MGS
Corn, Poison Jam, The Immortals - JSRF/JGR
Clement - Shadow Madness
Eddie - Iron Maiden: Ed Hunter
Turret - Portal

The whole friggin' cast from Guyilty Gear. That series has some awesome characters.

Especially i-NO